New Batman film The Dark Knight Rises sets a record
But the blockbuster’s takings were below industry forecasts made before the mass shooting at a midnight showing of the film in Colorado on Friday.
Projected figures suggested the film would make an estimated $173m (£111m), based on Friday receipts.
Twelve people were killed and 58 hurt in the attack at a cinema in Aurora.
The top two highest-ever openings were both 3-D films – The Avengers with $207m (£133m) and Harry Potter and the Deathly Hallows Part 2 with $169m (£109m).
Following the shooting, US cinemas tightened security and Warner Bros scaled down its promotional plans.
A premiere in Paris was cancelled, along with personal appearances by the cast and crew in Japan and Mexico.
The third and final film in director Christopher Nolan’s Batman series stars Christian Bale and Anne Hathaway. It cost Warner Bros $250m (£161m) to make.
Studios including Sony, Fox, Disney, Paramount and Warner Bros publicly withdrew their usual official revenue reports out of respect for the victims and their families, but sources gave details of the takings to media outlets.
Last week’s number one, Ice Age: Continental Drift, dropped to second place, with The Amazing Spider-Man in third.